ABSTRACT
AIM To elucidate the structure of the glycan of SPPA-1, a glycoconjugate isolated from Spirulina platensis. METHODS Methylation analysis, GC/MS, and 1D, 2DNMR techniques were used to determine the structures of the glycoconjugate (SPPA-1). RESULTS SPPA-1 was only composed of α-D-glucose and shown to be a (1→4) linked α-D-glucan to which a few glucosyl side chains are attached at O-6 of the glucosyl residues of the main chain. CONCLUSION The glycan of SPPA-1 is a new glucan.
ABSTRACT
A polysaccharide SPPC 1 was isolated from spirulina platensis by acetone fractional precipation, free protein was removed by trichloroacetic acid(TCA). Purification of the polysaccharide by Sephadex G 75 colume and CM Sephadex C 50 colume.The homogeneity of this polysaccharide was proved by HPLC and CE. molecular weight was 1.62?10 6. GC showed that the glycoconjugate was composed of Rha,Xyl,Man and Glc. Molar ratio is Rha:Xyl:Man:Glc=6.24:0.37:0.13:0.71. IR, 1HNMR spectrum indicated the presence of ? linkage glycoside. ESR experiment showed that SPPC 1 could eliminate O . 2 radicals.